基因组测序技术 略记

Workflow of NGS 二代测序流程

1. Sample fragmentation
2. Library preparation
3. Clonal Amplification
4. Sequencing reaction
5. Data analysis

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Principle of SMRT

1. Millions of zero-mode waveguides (ZMWs), embedded with only one set of enzymes and DNA template that can be detected during the whole process.
2. During the reaction, the enzyme will incorporate the nucleotide into the complementary strand and cleave off the fluorescent dye previously linked with nucleotide.
3. The camera inside the machine will capture signal in a movie format in real-time observation.

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整理自MOOC