带有DNA结构锁的三磷酸腺苷响应金属-有机框架，用于构建比例SERS生物传感器
Nowadays,metal organic frameworks (MOFs) are often widely applied in gas storage,20 organic matter adsorption,21 metal ion detection,22 and protein detection23 due to their large specific surface area, highly ordered topological structures, excellent biocompatibility and high porosity.24–26
如今，由于金属有机骨架（MOF）的比表面积大，拓扑结构高度有序，生物相容性好和高孔隙率，因此通常广泛应用于储气，20有机物吸附，21金属离子检测，22和蛋白质检测23。 –26最近，MOF逐渐成为构建生物平台的载体材料。 27–29最有代表性的是，Willner等。
Recently, MOFs have emerged as carrier materials for constructing biological platforms. 27–29 Most representatively, Willner et al. assembled a series of stimuli-responsive DNA-functionalized MOFs as drug carriers in cancer cells.30
装配了一系列刺激反应的DNA功能化MOF作为癌细胞中的药物载体。30 O. Farha等。合成了Zr-MOF作为纳米酶载体，可促进神经毒剂的水解。
O. Farha et al. synthesized a Zr-MOF as a nanosized enzyme carrier for accelerating the hydrolysis of nerve agents.31 In this study, DNA functionalized MOFs were employed for loading Raman reporter molecules (TB) inside. In the presence of ATP, theDNA structure on the surfaces of the MOFs was disassembled to release toluidine blue (TB) and complementary DNA (cDNA) for obtaining the Raman signal of TB. In this strategy, a SERS biosensor was fabricated based on theDNAstructure functionalized MOFs.
首先，通过修饰MOF表面的DNA Y结将TB包裹在微孔中。32,33三元Y结由ATP适配子（S2），cDNA和单链DNA（S1）组成。如方案1所示，在ATP存在下，ATP适体与ATP结合以分解Y-结结构，因此释放了大量的TB和cDNA。之后，将含有TB和cDNA的溶液滴在带有发夹DNA（H1）的GNF上，该cDNA与H1互补（用Cy5标记）在底物上形成刚性双链DNA（dsDNA）。结果，结核病
Firstly, TB was encapsulated in themicropores bymodifying the DNA Y-junctions on the surfaces of the MOFs.32,33 The ternary Y-junction consisted of an ATP aptamer (S2), cDNA and single stranded DNA (S1). As shown in Scheme 1, in the presence of ATP, the ATP aptamer combined with ATP to disintegrate the Y-junction structure, so large amounts of TB and cDNA were released. After that, when the solution containing TB and cDNA was dropped on the GNFs with hairpin DNA (H1), the cDNA complemented with H1 (labeled with Cy5) to form rigid double-stranded DNA (dsDNA) on the substrate. As a result, TB was
31在这项研究中，DNA功能化的MOF被用于在内部装载拉曼报告分子（TB）。在ATP存在下，MOF表面上的DNA结构被分解以释放甲苯胺蓝（TB）和互补DNA（cDNA）以获得TB的拉曼信号。在此策略中，基于DNA结构功能化的MOF制备了SERS生物传感器。
