报错内容
Failed to close filehandle AMBIG_1: Bad file descriptor at /data/bin/bismark line 2641, <IN2> line 34189156.
Failed to close filehandle AMBIG_2: Bad file descriptor at /data/bin/bismark line 2642, <IN2> line 34189156.
Failed to close filehandle UNMAPPED_1: Bad file descriptor at /data/bin/bismark line 2643, <IN2> line 34189156.
Failed to close filehandle UNMAPPED_2: Bad file descriptor at /data/bin/bismark line 2644, <IN2> line 34189156.
原作者回答
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To a) I think this is just a warning message about closing file handles, you should just ignore the message (did you even report amibiguous or unmapped reads?)
忽略即可
