内容来自视频，网址附后
主要内容如下
Tissue Prepartaion and Compatibility
https://support.10xgenomics.com/spatial-gene-expression/tissue-optimization/doc/specifications-visium-spatial-gene-expression-optimized-tissues
Sequencing
Minimum Sequencing Depth: We recommend a minimum of 50k read pairs per spot covered with tissue. To calculate this, first estimate the % of capture area covered by the tissue section based upon the H&E brightfield image. Then use the following formula to calculate the recommended sequencing depth:
• Total sequencing depth = (Coverage Area x total spots on the Capture Area) x 50,000 read pairs/spot
Example calculation for 60% tissue coverage: (0.60 x 5,000 total spots) x 50,000 read pairs/spot = 150 million total read pairs for that sample
Additional guidelines for estimating the number of spots covered: Determining number of spots covered with tissue may be performed visually or by using the Visium Manual Alignment Wizard in Loupe Browser for a more accurate measurement.
https://support.10xgenomics.com/spatial-gene-expression/sequencing/doc/specifications-sequencing-requirements-for-visium-spatial-gene-expression
https://support.10xgenomics.com/spatial-gene-expression/software/pipelines/latest/what-is-space-ranger
