Interpretation of Sequencing Chromatograms的笔记

https://brcf.medicine.umich.edu/cores/advanced-genomics/faqs/sanger-sequencing-faqs/interpretation-of-sequencing-chromatograms/

1. GET A GENERAL SENSE OF HOW CLEAN THE SEQUENCE IS
   How clear are the nucleotide peaks, in general?

2. CHECK FOR MIS-CALLED NUCLEOTIDES
   Are there obvious errors in the base calls?

3. LOSS OF RESOLUTION LATER IN THE GEL
   Even normal chromatograms stop giving accurate data after some distance

4. TRUNCATE THE SEQUENCE WHEN PROBLEMS BECOME TOO FREQUENT FOR YOUR PURPOSES
   What data quality do you need, in order to accomplish your goals?