改软件可快速去除PCR重复，较samtools，picard更快
安装
点击下载后，减压缩即可
http://lomereiter.github.io/sambamba/docs/sambamba-markdup.html
简单应用
参数
Usage: sambamba [command] [args...]
Available commands:
view view contents and convert from one format
to another (SAM/BAM/JSON/UNPACK)
index build index (BAI)
merge merge files (BAM)
sort sort file (BAM)
slice slice file (BAM using BED)
markdup mark or remove duplicates (BAM)
subsample subsample (BAM)
flagstat output statistics (BAM)
depth output statistics (BAM)
validate simple validator (BAM)
和samtools一些功能类似，着重看下markdup功能，及去除重复
Usage: sambamba-markdup [options] <input.bam> [<input2.bam> [...]] <output.bam>
By default, marks the duplicates without removing them
Options: -r, --remove-duplicates
remove duplicates instead of just marking them
-t, --nthreads=NTHREADS
number of threads to use
-l, --compression-level=N
specify compression level of the resulting file (from 0 to 9)
-p, --show-progress
show progressbar in STDERR
--tmpdir=TMPDIR
specify directory for temporary files
应用也较为简单，可以通过‘-r’参数，设置标记该重复或者去除该重复
