Lecture 1 -Scope and Overview
整体上介绍系统生物学,以及后面需要考虑的内容
设计系统生物学实验需要考虑的问题:
Lecture 2 - Biological Model Systems
讲述了用于建模的一些细胞和动物模型。
What biological system?
• E.g. Do I look at human cell lines, a mouse, or yeast?
Lecture 3 - Experimental Perturbations实验扰动
基因敲除技术、遗传筛选、iRNA
What perturbation/treatment conditions?
• E.g. What compounds should I apply to the system to elicit 引起a relevant response?
Lecture 4 - Measuring Nucleic Acids核酸
What measurements?
• E.g. What transcripts do I need to look at, and/or do I need to look at protein
levels instead?
介绍了测量核酸表达量的一些技术
Lecture 5 - Measuring Protein and Protein States
介绍了测量蛋白表达量的一些技术