1.预测理化性质:分子量(Da)、等电点(PI)\不稳定系数(Instability index)、亲水指数(Grand average of hydropathicity-简写GRAVY)、脂溶指数(Aliphatic index)
ProtParam tool (https://web.expasy.org/protparam/)
2.预测跨膜结构域
TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/)
结果解读:http://www.genedenovo.com/news/582.html
3.预测信号肽
SignalP-5.0(http://www.cbs.dtu.dk/services/SignalP/)
结果解读:http://www.cbs.dtu.dk/services/SignalP-3.0/output.php
4.蛋白质亚细胞定位预测
BUSCA(http://busca.biocomp.unibo.it/)
5.预测二级结构
SOPMA (https://npsa-prabi.ibcp.fr/NPSA/npsa_sopma.html)
6.预测三级结构
SWISS-MODEL(https://swissmodel.expasy.org/)
I-TASSER(https://zhanglab.ccmb.med.umich.edu/I-TASSER/)
phyre2(http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index)