在开始对比时，使用如下参数
STAR \
--genomeDir STAR \
--runThreadN 39 \
--readFilesIn /raid1/wzw/Ensete/hisat/Ensete_RNA_1.fq /raid1/wzw/Ensete/hisat/Ensete_RNA_2.fq \
--readFilesType Fastx\
--outFileNamePrefix ECLrna_ \
--outSAMtype BAM SortedByCoordinate \
--outBAMsortingThreadN 39 \
--outSAMstrandField intronMotif \
--outFilterIntronMotifs RemoveNoncanonical
出现如下错误
BAMoutput.cpp:27:BAMoutput: exiting because of OUTPUT FILE error: could not create output file ECLrna_STARtmp//BAMsort/8/80
SOLUTION: check that the path exists and you have write permission for this file. Also check ulimit -n and increase it to allow more open files.
阅读错误发现应该是bamsort的时候会使用大量进程，导致超过了最大线程数调整--outBAMsortingThreadN 参数到5
--outBAMsortingThreadN 39
终于正常运行了
