创建录制宏:打开一个图片 --- Plugins --- Macros --- Record --- name it
格式转化:Image --- Type --- 8-bit(为灰度图片)
增强对比度:Process --- Enhance contrast (选“normalize”+"0.3%")
平滑处理:Process --- Smooth
寻找边缘:Process --- Find Edges
调节阈值:Image --- Adjust --- Threshold(分别为“0” “20”)--- Apply
魔棒工具:使用魔棒工具选择划痕区域
划痕面积:Analyze --- Measure
保存录制宏:Create --- file --- save --- 图片所在的文件夹
伤口愈合百分比(Wound healing percentage):(0h面积-xh面积)/ 0h面积
ImageJ处理划面积:https://www.bilibili.com/video/BV13b41177ny/