（陈玲玲老师团队的ceRNA分析）

Introduction

Inorganic phosphate (Pi) is essential for the growth and productivity of plants；
The competing endogenous RNA (ceRNA) theory has been proved and is now acknowledged widely；
lincRNAs compete with genes to play important roles in rice undergoing Pi starvation.

Methods

RNA-seq data
数据质控：quality control (QC) toolkit
比对到参考基因组：tophat tool
合并重复：Samtools
组装：Cufflinks
最终组装：Cuffmerge
间隔区的选择：Cuffcompare
蛋白编码能力预测：Coding Potential Assessment Tool (CPAT) 和Coding Potential Calculator

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