免疫细胞:PRPTC 上皮细胞 epithelial cells:EPCAM
T细胞,T cell:
通用marker:CD3D,CD3E,
CD4+T细胞:CD4;CD8+T细胞:CD8A,CD8B;Treg:CD4,FOXP3
还有注意γδT细胞
B细胞,B cell:
CD79A,CD79B
中性粒细胞,Neutrophils:
FCGR3B,CXCR2
http://www.360doc.com/content/21/0622/12/19913717_983164617.shtml
DC细胞:
cDC1(human:XCR1+ CLEC9A+ CD141+):交叉递呈抗原给CD8+T细胞,
cDC2(CD11b+ SIRPα+
CD1c+):激活CD4+ T 细胞和CD8+T细胞。
浆细胞样DC (plasmacytoid
DC,pDC)(CD123+ CLEC4C+.)
参考:
NK细胞:
CD56(NCAM1),CD16(FCGR3A)
人类NK细胞表面标志主要以CD16、CD56来认定。目前多以CD3-、CD16+、CD56+作为NK细胞典型标志。
FGFBP2, FCG3RA, CX3CR1(有些文献使用这些marker)
巨噬细胞 macrophage:
CD68/CD163等。M1和M2型不太好分,更多marker可以参照赛默飞网站写的说明。https://www.thermofisher.cn/cn/zh/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work/macrophage-cell-overview.html
星型胶质细胞,astrocytes:
AQP4, ADGRV1, GPC5,RYR3
小胶质细胞,microglia:
C3, LRMDA, DOCK8
内皮细胞 endothelial cells:
VWF,FCN3,DNASE1L3,
肥大细胞 Mast cell:
TPSAB1 ,TPSB2,CPA3,KIT
成纤维细胞,fibroblast :
FGF7, MME, DCN,LUM, GSN,DCN,ACTA2,FAP,PDGFRA
肌成纤维: MMP11、POSTN、CTHRC1、COL1A1、ACTA2 和 COL3A1(参考:https://www.cn-healthcare.com/articlewm/20230822/content-1595535.html)
Fibroblasts were identified by their
archetypal markers LUM, DCN, VIM, PDGFRA, and COL1A2 (参考:https://www.jianshu.com/p/5a1b1283b52e?utm_campaign=haruki)
周细胞,pericyte :
RGS5,DCN, LUM ,
GSN ,FGF7,MME, ACTA2
HSC(hematopoietic stem cells):
黑色素肿瘤细胞:
Tumor cells were identified usingMLANA,
MITF,and DCT. Tumor cells were further divided into subgroups by expressionofPRAME and GEPgenes("Single-cell analysis reveals new
evolutionary complexity in uveal melanoma")