脂肪相关-qPCR基因-文献参考

• Cladosporols A 和 B 是两种天然过氧化物酶体增殖物激活受体γ（PPARγ）激动剂，可抑制 3 T3-L1 前脂肪细胞的脂肪形成，并导致 HT-29 细胞增殖的条件培养基依赖性停滞- ScienceDirect --- Cladosporols A and B, two natural peroxisome proliferator-activated receptor gamma (PPARγ) agonists, inhibit adipogenesis in 3T3-L1 preadipocytes and cause a conditioned-culture-medium-dependent arrest of HT-29 cell proliferation - ScienceDirect

As expected, expression of the two early adipogenesis-related genes, PPARγ and C/EBPα, was significantly reduced after treatment with MDI plus 5 μM cladosporol A or cladosporol B for 3 days (Fig. 3B). Furthermore, the mRNA level of four of terminal differentiation markers (aP2, FASN, adiponectin and perilipin) was diminished when the cells were treated with MDI plus 5 μM cladosporol A or cladosporol B for 10 days, compared with cells treated with MDI or MDI plus RGZ (Fig. 3C).

两种早期脂肪生成相关基因 PPARγ和 C/EBPα的表达显著降低（ 图 3B）。此外，与用 MDI 或 MDI 加 RGZ 处理的细胞相比，当用 MDI 加 5μM 枝孢酚 A 或枝孢酚 B 处理细胞 10 天时，四种终末分化标志物（aP 2、FXR、脂联素和围脂蛋白）的 mRNA 水平降低