该方法出自于1991年K.Edwards在Nucleic Acids Research发表的名为A simple and rapid method for the preparation of plant genomic DNA for PCR analysis的文章，亲测好用，对于DNA质量要求不高的试验可极大地提高试验效率。
下面是文章核心内容：
DNA is extracted as follows : The tissue is macerated ( using disposable grinders from Bel - art Products : Scienceware , Pequannock , NJ ,07440 USA . catalog no 992) in the original Eppendorf tube at room temperature , without buffer , for 15 seconds .400μL of extraction buffer (200 mM Tris HCl pH 7.5,250 mM NaCl ,25 mM EDTA ,0.5% SDS ) is added and the sample vortexed for 5 seconds . This mixture can then be left at room temperature until all the samples have been extracted (>1 hour ). The extracts are centrifuged at 13,000 rpm for 1 minute and 300 ul of the supernatant transferred to a fresh Eppendorf tube . This supernatant is mixed with 300μ l isopropanol and left at room temperature for 2 minutes . Following centrifugation at 13,000 rpm for 5 minutes , the pellet is vacuum dried and dissolved in 100 ul 1xTE. This DNA is stable at 4 ℃.
