Week 1_08_GenomicRanges - GRanges
# source("http://www.bioconductor.org/biocLite.R")
# biocLite(c("GenomicRanges"))
library(GenomicRanges)
gr <- GRanges(seqnames = "chr1",
strand = c("+", "-", "+"),
ranges = IRanges(start = c(1, 3, 5), width = 3))
## 获得下游区域
flank(gr, 2, start = FALSE)
## 获得上游2bp区域
flank(gr, 2, start = T)
## seqinfo
seqinfo(gr)
seqlengths(gr) <- c("chr1" = 10)
seqinfo(gr)
seqlevels(gr)
# gaps 获取基因上没有倍GRanges覆盖到的区域
gaps(gr)
seqlevels(gr) <- c("chr1", "chr2")
seqnames(gr) <- c("chr1", "chr2", "chr1")
seqinfo(gr)
sort(gr)
seqlevels(gr) <- c("chr2", "chr1") # 定义染色体的水平顺序
sort(gr) # 排序后会按照指定的染色体顺序来排序
genome(gr) <- "hg19" ## 注明基因组的版本
gr
gr2 <- gr
genome(gr2) <- "hg18"
findOverlaps(gr, gr2) ## 由于基因组版本号不同所以结果会报错
GRanges 的参考说明书学习
# 新版本包安装方法
if (!require("BiocManager"))
install.packages("BiocManager")
BiocManager::install("GenomicRanges")
library(GenomicRanges)
gr <- GRanges(seqnames = Rle(c("chr1", "chr2", "chr1", "ch3"), c(1, 3, 2, 4)), # 目前理解Rle这里就相当于rep函数
ranges = IRanges(101:100, end = 111:120, names = head(letters, 10)), # 指定区域坐标，以及每个区间的名称
strand = Rle(strand(c("-", "+", "*", "+", "-")), c(1, 2, 2, 3, 2)),
score = 1:10,
GC = seq(1, 0, length = 10)
)
seqnames(gr)
ranges(gr)
strand(gr) ## 对于重复数据比较多的情况使用Rle能后节省空间
granges(gr)
gr
mcols(gr) # 提取metadata列
mcols(gr)$score
seqlengths(gr) <- c(249250621, 243199373, 198022430) ## 指定每条染色体的长度
seqlengths(gr)
names(gr)
length(gr)
# 2.1 分割和合并GRanges
sp <- split(gr, rep(1:2, each = 5)) # 分割
sp
c(sp[[1]], sp[[2]]) # 合并
# 2.2 筛选GRanges
gr[2:3]
gr[2:3, "GC"]
singles <- split(gr, names(gr))
singles
grMod <- gr
grMod[2] <- singles[[1]]
head(grMod, n = 3)
## 重复、反转、选择
rep(singles[[2]], times = 3)
rev(gr)
head(gr, n = 2)
tail(gr, n = 2)
window(gr, start = 2, end = 4)
gr[IRanges(start = c(2, 7), end = c(3, 9))] ## 结合IRanges来筛选目标行
# 2.3 Basic interval operations for GRanges objects
g <- gr[1:3]
g <- append(g, singles[[10]]) ## append函数表示在末尾添加信息
start(g)
end(g)
width(g)
range(g)
flank(g, 10) # 获取下游downstream区域10
flank(g, 10, start = F) ## 获取上游Updtream区域10
shift(g, 10) ## 表示整体向前移动十个单位
resize(g ,10) ## 将区间重新定义，保持起始位置不变，将其变为宽度为10的区间（考虑了正负方向）
reduce(g) ## 将交集区域合并为一个区域
gaps(g) ## 取基因组上没有被覆盖的区域
disjoin(g) ## 取已有区域没有交集的区域坐标
coverage(g) ## 将所有区域按照交集程度划分为不同等级
# 2.4 Interval set operations for GRanges objects
g2 <- head(gr, n = 2)
union(g, g2) ## 取两个GRanges的并集
intersect(g, g2) # 取交集区域
setdiff(g, g2) # 取没有交集的区域
g3 <- g[1:2]
ranges(g3[1]) <- IRanges(start = 105, end = 112) ## 修改区域
punion(g2, g3) ## 当有相同的metdata时候，可以使用p开头的来进行操作
pintersect(g2, g3)
psetdiff(g2, g3)
methods(class = "GRanges") # 查看GRanges里面所有函数
# 3GRangesList: Groups of Genomic Ranges
gr1 <- GRanges(
seqnames = "chr2",
ranges = IRanges(103, 106),
strand = "+",
score = 5L, GC = 0.45)
gr2 <- GRanges(
seqnames = c("chr1", "chr1"),
ranges = IRanges(c(107, 113), width = 3),
strand = c("+", "-"),
score = 3:4, GC = c(0.3, 0.5))
grl <- GRangesList("txA" = gr1, "txB" = gr2)
# 3.1 Basic GRangesList accessors
seqnames(grl)
ranges(grl)
strand(grl)
length(grl)
names(grl)
seqnames(grl)
elementNROWS(grl) # 统计每一个小GRanges的行数
isEmpty(grl)
mcols(grl) <- c("Transcript A", "Transcript B")
mcols(unlist(grl))
# 3.2 Combining GRangesList objects
ul <- unlist(grl)
ul
grl1 <- GRangesList(
gr1 = GRanges("chr2", IRanges(3, 6)),
gr2 = GRanges("chr1", IRanges(c(7,13), width = 3)))
grl2 <- GRangesList(
gr1 = GRanges("chr2", IRanges(9, 12)),
gr2 = GRanges("chr1", IRanges(c(25,38), width = 3)))
gr1
gr2
pc(grl1, grl2) ## 合并两个GRanges
grl3 <- c(grl1, grl2)
regroup(grl3, names(grl3)) ## 这两步等同于pc()
# 3.3 Basic interval operations for GRangesList objects
start(grl)
end(grl)
width(grl)
sum(width(grl))
shift(grl, 20)
coverage(grl)
# 3.4 Subsetting GRangesList objects
grl[1]
grl[[1]]
grl["txA"]
grl$txB
grl[1, "score"]
grl["txB", "GC"]
rep(grl[[1]], times = 3)
rev(grl)
head(grl, n = 1)
tail(grl, n = 1)
window(grl, start = 1, end = 1)
grl[IRanges(start = 2, end = 2)]
# 3.5 Looping over GRangesList objects
lapply(grl, length)
sapply(grl, length)
grl2 <- shift(grl, 10)
names(grl2) <- c("shiftTxA", "shiftTxB")
mapply(c, grl, grl2)
Map(c, grl, grl2)
endoapply(grl, rev)
mendoapply(c, grl, grl2)
Reduce(c, grl)
gr <- unlist(grl)
gr$log_score <- log(gr$score)
grl <- relist(gr, grl)
# 更多资料
?GRangesList
methods(class="GRangesList") # _partial_ list
# 4 Interval overlaps involving GRanges and GRangesList objects
findOverlaps(gr, grl)
countOverlaps(gr, grl)
subsetByOverlaps(gr,grl)
findOverlaps(gr, grl, select="first")
findOverlaps(grl, gr, select="first")
