1,Phagocytic cells pecentage(具有吞噬作用的细胞比例):the percentage of phagocytic cells is the number of macrophages that ingest at least one tumor cell divided by the total number of macrophages,如图1.1。
2,Phagocytic index(吞噬指数):phagocytic index is the number of ingested tumor cells divided by the total number of macrophages. 也就是总共被吞的tumor cells个数除以总巨噬细胞数目(number of phagocytized tumor cell per macrophage的意思)
3,Mean fluorescent intensity(平均荧光强度,MFI):无论是基于FACS还是荧光显微镜成像,tumor cell的荧光强度和个数的加权平均,MFI和Phagocytic index有近乎完美的correlation,如下图1.2。
【Reference】
1,Alveolar macrophage phagocytosis and bacterium clearance in mice. Journal of Visualized Experiments,2019.
2,Budesonide but not terbutaline decreases phagocytosis in alveolar macrophages.Respiratory Medicine, 1998.