2020.02.04文献
- 1. 3,3-Dimethyl-1-butanol attenuates cardiac remodeling in pressure-overload-induced heart failure mice. by Guangji Wang, Bin Kong, …, He Huang (2020) J. Nutr. Biochem. (impact factor: 4.5) 2 区
P (研究人群/动物) C57BL6/J male mice (8-10 weeks old)
I（干预措施) AB+with or without 1% DMB for 6 weeks
C (对照措施) Sham+with or without 1% DMB for 6 weeks
O (结果)Cardiac function, plasma TMAO level, cardiac hypertrophy and fibrosis, expression of inflammatory, susceptibility to ventricular arrhythmia and p65 NF-κB and TGF-β1/Smad3 signaling pathway were prevented by DMB treatment at the 6th week after AB surgery
思考
- 心功能只有射血分数、血液动力学指标缓解了,但是病理指标都缓解了，可靠否？
- 机制也是只观察了现象
- 没有细胞实验
- 2. DNMT1 Methylation of LncRNA GAS5 Leads to Cardiac Fibroblast Pyroptosis via Affecting NLRP3 Axis. by Qian She, Peng Shi, …, Yan Yang (2020) Inflammation (impact factor: 2.9)3 区
P In Vivo: Sprague-Dawley rats weighing 180 to 200 g; In Vitro: SD rats of 3–5 days old
I In Vivo: ISO 5 mg/kg, for 2 weeks;In Vitro: 1 μg/ml of LPS for 5.5 h, and then cultured for 5 h by adding 5 ng/ml of ATP
C In Vivo: normal saline for 2 weeks; In Vitro :PBS
O DNMT1 methylation silencing of LncRNA GAS5 could lead to cardiac fibroblast pyroptosis
and activated the NLRP3, thus promoting the progression of cardiac fibrosis.
思考
- LncRNA与甲基化就是一个siRNA和一个甲基酶抑制剂就证明有相互关系了吗？
- 敲低或者过表达 LncRNA GAS5 的时候为什么没有LPS+敲低或者过表达组,只有Control NC Si/PcNDA组?
- 3. Inhibiting SLC26A4 reverses cardiac hypertrophy in H9C2 cells and in rats. by Liqun Tang, Xiaoqin Yu, Yangyang Zheng, Ning Zhou (2020) PeerJ (impact factor: 2.4) 3 区
P In Vivo:ten healthy male Sprague Dawley (SD) rats weighed 250 ± 20 g In Vitro: H9C2 cells (an embryonic rat myocardium-derived cell line)
I In Vivo: abdominal aortic constriction (AAC)+injected with or without siRNA-SLC26A4 (2.5 ul) once a day for seven days.; In Vitro:PE (200 mM) for 48 h+SLC26A4 siRNA or the control siRNA
C In Vivo: Sham+ normal saline for for seven days; In Vitro :PBS?++SLC26A4 siRNA or the control siRNA
O Inhibiting SLC26A4 could decrease the release of ANP or BNP and promote the expression of GSK-3beta in vivo and in vitro.
思考
- 动物试验中没有sham+siRNA组
- 动物试验中没有心功能缓解的情况.只有个Mrna和病理缓解情况
- 结论中"SLC26A4 increases cardiac hypertrophy,"文中并没有做过表达实验,如何得到这个结论?
- GSK-3beta这个指标能够提到beta-MHC?
- 4. The Pro-arrhythmic Features of Pathological Cardiac Hypertrophy in Neonatal Rat Ventricular Cardiomyocyte Cultures. by Zeinab Neshati, Martin Schalij, Antoine de Vries (2020) J. Appl. Physiol.
跟我们的研究好像没啥关系,没看了
PS
