Blast在线比对（2018-05-31）

用提取出的基因去blastn在线比对refseq_rna库
参数设置如下：
Optimize for Somewhat similar sequences (blastn)
Max target sequences：100
Expect threshold：10
Word size：11
其他的参数选择默认

结果报错：

• There was a problem with the search. Please, contact Help Desk and include RID GYJ13SBR01R

  CPU usage limit was exceeded. You may need to change your search strategy. Helpful changes include reducing the number of queries, choosing a smaller database and setting an organism limit. You may also need to adjust the Algorithm parameters in the bottom section of the form. Choose a smaller number of target sequences, set a smaller Expect cut-off, use a larger word-size and turn on species specific repeats.

• 超出了CPU使用限制。您可能需要更改搜索策略。有用的更改包括减少查询次数、选择较小的数据库和设置有机体限制。您还可能需要调整窗体底部部分的算法参数。选择较小数量的目标序列，设置较小的预期截止值，使用更大的字大小并开启物种特定重复。

报错界面.png

将word size调整到7，重新跑，遇到类似问题，需要进一步调整。