Cell hashing是一种在单细胞rna测序平台上实现样本多线程和超负载的方法,由纽约基因组中心的技术创新实验室与Satija实验室合作开发。
Cell Hashing uses a series of oligo-tagged antibodies against ubiquitously expressed surface proteins with different barcodes to uniquely label cells from distinct samples, which can be subsequently pooled in one scRNA-seq run. By sequencing these tags alongside the cellular transcriptome, we can assign each cell to its sample of origin, and robustly identify doublets originating from multiple samples.