一天的工作结束了，今天过的很好。因为没有值班，所以一天的时间都花在文献阅读上面。在昨天的基础上看完了一篇文献，并且按照网上搜索的方法进行做了笔记。以前都是用本子来记，但今天没有，而是见了一个word的文档，并命名为《文献与读笔记》，希望这个文档能坚持续写几年。在此一在这里将复制一下今天的笔记，一此来纪念新的阅读方式。
title 《Association study of gut flora in coronary heart disease through high-throughput sequencing》
Introduction
Although the diversity of human gut microbiota has been observed in different individuals, the unique core of its composition is stable, suggesting a potiental risk of intestinal microbiota for human disease. Relationships between gut microbiota and cardiovascular disease have attracted more and more attentions. Oral dietary supplementation with L-oxide by gut microbiota, has been proved to accelerate atherosclerosis in mice. Then, high-throughput sequencing was used to assess the taxonomic composition of the microbiota in CHD patients.
Materials and methods
Between April 2015 and June 2015,29 CHD in hospital patients were recruited and 35 healthy volunteers were recruited as control. The inclusion criteria as follows (1,2,3). Fresh fecal samples(2-5g) were obtained from all the patients under the hospital diet and controls under the usual diet.
Results
3.1 baseline characteristics of patients. Age, sex, BMI, hypertension.
3.2 high-throughput sequencing data analysis.
3.3 OTU analysis. venn picture and rank-abundance curve of operational taxonomic units in the groups.
3.4 specials classification. Reads corresponding to 38 phyla, 90 classes, and 584 genera were detected in CHD patients fecal samples.
3.5 analysis of a Diversity index. a diversity of the CHD patients microbiota was significantly higher than that of. The controls. Chao 1 index, and Ace index in the CHD patients were higher than those in the control group.
3.6 Intestinal flora structure analysis.
3.7 PcoA and UPGMA TREE analysis. Samples contribution rates of the first the PcoA second PcoA(PC2), and third PcoA (PC3) were 11.85%,4.09%,and 2.84% respectively, which highlighted a clear clustering of the microbial population of the CHD patients away from that of the controls.
DISCUSSION
Obesity is one of the major risk factors for CHD, as the process from overweight to obese brings in a large number of comorbidities, which are harmful for cardiovascular health. As an independent maker of the risk of cardiovascular disease, blood proteobacteria has been verified to be positively related to the onset of cardiovascular complications. Same limitations. First, the samples size of patients was small. Second, it is unclear whether the differences of gut flora compositions between groups were response to CHD or they actively inducted the CHD.
明天后天两天将会休息，而明天将准备骑着共享单车去40公里之外的响水涧看看油菜花，当然这一切是在明天有个好天气的情况下。期待明天的独自旅行。
