Xiao Y, Cai D, Yang W, et al (2012) Genetic structure and linkage disequilibrium pattern of a rapeseed (Brassica napus L.) association mapping panel revealed by microsatellites. Theor Appl Genet 125:437–447. doi: 10.1007/s00122-012-1843-5

中性检测

使用PopGene 1.31版软件（Yeh等人1999）中实施的Manly算法（1985年）对各组和亚组进行了Ewens-Watterson的中立性测试（Ewens 1972；Watterson 1978），以调查整个基因座的选择性中立性。这是为了测试观察到的纯合子（计算为等位基因频率的平方和）是否明显高于或低于中性预期下的模拟预期纯合子，并可以提示选择是否在人群中的特定基因座上进行（Ewens 1972；Watterson 1978）。

数据

/* Diploid alphabetic data of 1 populations each with varying records (genotypes) & 21 loci */
Number of populations = 1
Number of loci = 21
Locus name: AAT-1 AAT-2 AAT-3 ACO ADH DIA-1 DIA-3 EST-2 GDH G6P HA IDH MDH-1 MDH-2 MDH-3 MDH-4 PEP-1 PEP-2 PGI-2 PGM SPG-2

AA AA AA AA AA AA AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AB BB A3 AA AB BB AA AA AA AA AA AA AA AA AA AB AA AA
AA AA AA AA BC AC AA AB AB AB AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA BB CC AA BB AB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA AB AC AA BB AA AA AA AA AA AA AA AC AA AA AA AB AA
AA AA AA AB AB AC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
AB AA AA AA BC AC AA AB AB AA AA AA AA AB AA AB AA AA AA AA AA
AA AA AA AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA AC AA AA
AA AA AA AA AA BC AA AB AA AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AB BC BC AA BB AB AA AA AA AA AB AA AA AA AA AA AC AA
AA AA AA AB AC AB AA BB BB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA AB AC AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA AA BC AB AB AA AA AA AA AA AA AA AA AA AA AC AA AA
AA AA AA AB AA AC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA BB BB AA AA AA AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA AC AC AA BB AB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AB BB BC AA BB AA AC AA AA AA AA AA AA AA AA AC AD AA
AA AA AA AA AB BC AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA BB BC AA BB AA AA AA AA AA AA AA AB AA AA AA AC AA
AA AA AA AA AA BC AA AB AB AA AA AA AA AA AA BC AA AA AA AA AA
AA AA AA AA AB BC AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA BD BC AA AB AB AA AA AA AA AA AA AA AA AA AC AE AA
AA AA AA AB CC BB AA BB AA AA AA AA AA AA AA AA AA AA AA AB AA
AA AA AA AA BC BB AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AB CC BB AA AA AB AA AA AA AA AA AA AA AA AA AA AB AA
AA AA AA AA BB BC AA AB AB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA AB BC AA AA BB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AB AC AB AA BB AA AA AA AA AA AA AA AA AA AA AA AB AA
AA AA AA AB BC BB AA BB BB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AB AB AB AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA BB AB AA AA AA AA AA AA AA AA AA BB AA AA AA AA AA
AA AA AA AA AC BC AA BB AA AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AA CC BC AA AB AB AA AA AA AA AA AA AB AA AA AA AC AA
AA AA AA AA BB AC AA BB AA AA AA AA AA AA AA AA AA AA CC AA AA
AA AA AB AA BE BB AA BB AB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA AB BC AA AB AA AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA BB AA CC AA AB AB AA AA AA AA AA AA BC AA AA AA AA AA
AA AA AA AB AC BC AA BB BB AA AA AA AA AA AA AA AA AA AA AA AA
AA AA AA AB AA BB AA AC AB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AB BB AC AA BB AA AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA AC AC AA AA AB AA AA AA AB AA AA AB AA AA AA AA AA
AA AA AA AA AA AB AA AB AB AA AA AA AA AA AA AB AA AA AA AA AA
AA AA AA AA BC BC AA BB AB AA AA AA AA AA AA AB AA AA AA AC AA

popgene

加载数据

file→load data→co-dominant marker data→选择确定。

分析

co-dominant→diploid data：

勾选

结果

结果

The observed fixation indexes of all of the identified polymorphic SSRs fell within the 95% confidence interval of theoretical expectation, suggesting that the hypothesis for neutral selection could not be rejected for any of these loci.