细胞周期分析[流式，使用anti-KI-67、7-AAD胞内染色]：
7-AAD signal is proportional to DNA content
7-AAD信号：最右边的峰代表G2/M期细胞；最左边的峰代表G0/G1期细胞；两峰之间代表S期细胞
G0/G1细胞群中：Ki-67-为G0期细胞，Ki-67+为G1期细胞
anti-mouse CD8α mAb，clone 3D9的制备：
1.immunizing and boosting Armenian hamsters with recombinant CD8a-hIg fusion proteins
2.脾脏细胞与SP2/0细胞系融合，with PEG 1500（聚乙二醇）
3.select、clone、screen for mAb of 杂交瘤细胞
Hydrodynamic Delivery of DNA：
（transfection—the introduction of DNA into cells）
offers a simple, convenient means for the expression of significant levels of transgenes in the organs, especially the liver, of mice.
The hydrodynamics method involves the rapid injection of a large volume (approximately the blood volume) of a saline solution of DNA (often in the plasmids) into mice via the tail vein.【本文中6s内静脉注射1.5ml PBS】
Depending on the particular plasmid construct, gene expression can reach a maximum between 4–8 h after injection and the transgenes can remain transcriptionally active for more than 6 mo
the receptor array technology(寻找CD8a的配体):
1.编码full-length human membrane proteins的约6000个plasmids分别转染到384孔板中的293T细胞中
2.24h后，将20ng human CD8a-hIg fusion protein与5ng APC-conjugated anti-human IgG Fc 的混合物加入各个孔板中
3.12h后，在Mirrorball Fluorescence Cytometer上读取孔板数据信号。识别APC信号为阳性的孔（peak intensity and cell perimeter were used as cut-offs to detect APC+ cell）
