conda安装并运行
wget https://repo.anaconda.com/archive/Anaconda3-2021.05-Linux-x86_64.sh
bash Anaconda3-2021.05-Linux-x86_64.sh
给activate加权限，激活conda
cd anaconda3/bin
chmod 777 activate
source ./activate
python2的配置
python --version
Anaconda3采用python2进行，因此要将python转为python2
conda create --name python2 python=2.7 -c https://mirrors.ustc.edu.cn/anaconda/cloud/bioconda/ -y
切换到python2环境
source activate python2
配置conda channels
conda config --add channels r
conda config --add channels default
conda config --add channels conda-forge
conda config --add channels bioconda
conda config --set show_channel_urls yes
MultiQC安装
conda install multiqc -c https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda
下载fastq文件
prefetch SRR10338477
prefetch SRR10442162
cp SRR10338477.sra ~/Seqs/multiqc_test
cp SRR10442162.sra ~/Seqs/multiqc_test
cd ~/Seqs/multiqc_test
运用fastqc进行数据质量评价
fastqc SRR10338477_1.fastq.gz SRR10442162_1.fastq.gz SRR10338477_2.fastq.gz SRR10442162_2.fastq.gz
运行multiqc
multiqc .
ls
