1.获取给定DNA序列的反向互补序列:

思路：从一个文件中导入序列，然后将互补后的结果输出到另外一个文件中。先定义的一个字典： complement = {'C': 'G', 'G': 'C', 'T': 'A', 'A': 'T'}
然后 for i in list（seq）：
　　　　rev_dna += complement[i]
　　　　rev_dna = rev_dna[::-1]
　　print （rev_dna）
直接得到互补后的结果

n =  0
complement = {'A':'T','G':'C','C':'G','T':'A'}
rev_seq: str= ''
with open(r'/home/yt/PycharmProjects/test/1minia_result.txt', 'w') as f1:
    with open(r'/home/yt/PycharmProjects/test/1minia.contigs.fa', 'r') as f:
       for dna_seq in f:
           n +=1
           if n < 100:
               rev_seq = ''
               if dna_seq.startswith(">"):
                   f1.write(dna_seq)
               else:
                   dna_seq = list(dna_seq.strip())
                   for i in dna_seq:
                       rev_seq += complement[i]
                       rev_seq = rev_seq[::-1]
                   f1.write(rev_seq)
                   f1.write('\n')

2.把DNA转换成RNA

思路：将DNA序列中的T换成U就可以啦，直接import re，然后用sub就行啦

import re
dnaSeq = ''
with open('/home/yt/PycharmProjects/test/1minia.contigs.fa') as f:
    for line in f:
        line = line.rstrip()
        dnaSeq += line.upper()
rnaSeq1 = re.sub('T', 'U', dnaSeq)
rnaSeq2 = dnaSeq.replace('T', 'U')
print(rnaSeq1)
print(rnaSeq2)

3.提取指定ID的序列

思路：将序列存入字典，打开字典读取序列，利用字典提取指定ID序列

dict={}
fw = open('/home/yt/PycharmProjects/test/1minia.result2.txt', 'w')
fr = open('/home/yt/PycharmProjects/test/1minia.contigs.fa', 'r')
for line in fr:
    if line.startswith('>'):
        line = line.strip('\n')
        line = line.replace(' ', '')
        name = line
        dict[name] = ''
    else:
        dict[name] += line.replace('\n','')
fr.close()
for ID in dict.keys():
    if ID == '>14__len__4122':
        fw.write(ID + "\n" + dict[ID])
fw.close()

4.每行指定长度输出序列

思路：使用循环输出列表，利用 计数器控制输出数量，当输出到第十个，计数器归零，重新开始计数，print输出增加end参数可以控制输出后以什么结尾,这里使用range方法快速生成10-90的数字添加进list列表

results = list(range(10, 90))
n = 10  # 每10个数换一行
for i in range(len(results)):
    print(results[i], end=' ')
    if (i+1) % 10 == 0:
        print("\n") 

with open(r'/home/yt/PycharmProjects/test/1minia.result4.txt', 'w') as f1:
    with open(r'/home/yt/PycharmProjects/test/1minia.contigs.fa', 'r') as f:
      seq = ''
      for line in f:
                seq = seq.strip('\n')
                if line.startswith('>'):
                    seq = line
                    f1.write(seq)
                else:
                    seqlist = list(line)
                    seqlist = seqlist[0:20]
                    seq = "".join(seqlist)
                    f1.write(seq+'\n')
f.close

5.按照序列长度/名字排序

fasta = {}
f1 =  open('/home/yt/PycharmProjects/test/1minia.result5.txt', 'w')
f = open('/home/yt/PycharmProjects/test/1minia.contigs.fa', 'r')
sortseq = ''
for line in f:
    if line.startswith('>'):
        line = line.strip('\n')
        name = line + '\n'
        fasta[name] = ''
    else:
        fasta[name] = line
for key, value in fasta.items():
    seqsort = sorted(fasta.items(), key=lambda i:len(i[1]))
    for n in seqsort:
        for t in n:
            sortseq += str(t)
f1.write(sortseq)

6.RNA翻译为蛋白质

首先我们要建立一个dictionary将RNA codon table存进去，key为RNA 序列，value是对应的氨基酸。

import re
from collections import OrderedDict
codonTable = OrderedDict()
f1 = open('/home/yt/PycharmProjects/test/RNA.result','w')
f = open('/home/yt/PycharmProjects/test/RNA','r')
    for line in f:
        line = line.rstrip()
        lst = re.split('\s+', line)
        for i in [0, 2, 4, 6]:
            codonTable[lst[i]] = lst[i + 1]
rnaSeq = ''
with open('/home/yt/PycharmProjects/test/RNA', 'rt') as f:
    for line in f:
        line = line.rstrip()
        rnaSeq += line.upper()
aminoAcids = []
i = 0
while i < len(rnaSeq):
    codon = rnaSeq[i:i + 3]
    if codonTable[codon] != 'Stop':
        aminoAcids.append(codonTable[codon])
    i += 3
peptide = ''.join(aminoAcids)
f1.write(peptide)