解读人：小杜的生信筆記
Reading date: 2022.2.11
Journal: Horticulture Research
College of Horticulture and Forestry Science, Huazhong Agricultural University
Tile: Function and transcriptional regulation of CsKCS20 in the elongation of very-long chain fatty acids and wax bniosynthesis in citrus sinensis flavedo: Cskcs20在黄皮柑橘超长链脂肪酸延伸和蜡质生物合成中的作用和转录调控
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文献摘要解读 | 华中农业大学揭示CsKCS20参与柑橘果实蜡质特异性组分积累的分子机制 - 知乎 (zhihu.com)
20220211 | CsKCS20参与柑橘果实蜡质特异性组分积累的分子机制*
译文详细讲解：
•Cuticular wax on plant aerial surfaces plays a vital role in the defense against various stresses, and the genes related to wax metabolism have been well documented in several model plants.
译文：植物气生表面的角质层蜡质在抵御各种逆境中起着重要的作用，与蜡质代谢相关的基因已经在一些模式植物中得到很好的证实。
Cuticular wax on plant aerial surfaces：植物气生表面的角质层蜡质
•However, there is very limited research on the key enzymes and transcription factors (TFs) associated with carbon chain distribution and wax biosynthesis in citrus fruit
译文：然而，对于柑橘果实碳链分布和蜡质生物合成相关的关键酶和转录因子的研究很少。
be very limited research on :在.....研究很少
•In this study, an analysis of wax metabolites indicated that even carbon-chain (C24-C28) metabolites are the dominant wax components in citrus fruit, and a 3-ketoacyl-CoA synthase (KCS) family gene (CsKCS20) plays an important role in the carbon chain distribution during wax biosynthesis in a wax-deficient mutant (MT).
译文：本研究中对蜡质代谢物的研究表明，蜡质代谢产物 c24-c28是柑橘蜡质代谢的主要成分，3- 酮酰基辅酶 a 合成酶(3- 酮酰基辅酶 a 合成酶)家族基因 cskcs20在缺蜡突变体(MT)蜡质生物合成过程中的碳链分布中起重要作用。
indicated that: 表明
•Expression of CsKCS20 in yeast indicated that CsKCS20 can catalyze the biosynthesis of C22 and C24 very-long-chain fatty acids (VLCFAs).
译文：Cskcs20在酵母中的表达表明，cskcs20能够催化 c22和 c24超长链脂肪酸的生物合成
yeast: 酵母
•In addition, transcriptome and sequence analysis indicated that the differential expression of CsKCS20 between the wild-type (WT) and MT fruit can be partly attributed to the regulation of CsMYB96, which was further confirmed by yeast one-hybrid (Y1H) assays, electrophoretic mobility shift assays (EMSAs) and dual luciferase assays.
译文：此外，转录组和序列分析表明， CsKCS20 在野生型(WT)和 MT 果实之间的差异表达可能部分归因于对 csmyb96的调控，这一结果通过酵母单杂交(Y1H)试验、电泳迁移率变化试验(EMSAs)和双荧光素酶试验得到进一步证实。
be partly attributed to：部分原因归因于.....
•The functions of CsMYB96 and CsKCS20 in wax biosynthesis were further validated by heterologous expression in Arabidopsis.
译文：通过在 Arabidopsis 的异源表达，进一步验证了 csmyb96和 cskcs20在石蜡生物合成中的作用。
be further validated by: 通过....进一步得到证实
•In summary, this study elucidates the important roles of CsKCS20 and CsMYB96 in regulating VLCFA elongation and cuticular wax biosynthesis, which provides new directions for the improvement of citrus fruit wax quality in genetic breeding programs.
译文：综上所述，本研究阐明了 cskcs20和 csmyb96在调控 VLCFA 伸长和角质层蜡质生物合成方面的重要作用，为柑桔果实蜡质品质的遗传育种改良提供了新的方向。
In summary: 总上所述
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