Formative pluripotency: the executive phase in a developmental continuum - PubMed (nih.gov)
摘要
The regulative capability of single cells to give rise to all primary embryonic lineages is termed pluripotency. Observations of fluctuating gene expression and phenotypic heterogeneity in vitro have fostered a conception of pluripotency as an intrinsically metastable and precarious state. However, in the embryo and in defined culture environments the properties of pluripotent cells change in an orderly sequence. Two phases of pluripotency, called naïve and primed, have previously been described. In this Hypothesis article, a third phase, called formative pluripotency, is proposed to exist as part of a developmental continuum between the naïve and primed phases. The formative phase is hypothesised to be enabling for the execution of pluripotency, entailing remodelling of transcriptional, epigenetic, signalling and metabolic networks to constitute multi-lineage competence and responsiveness to specification cues
单细胞产生所有初级胚胎谱系的调节能力称为多能性。对波动的基因表达和体外表型异质性的观察产生了多能性作为一种内在亚稳态和不稳定状态的概念。然而，在胚胎和特定的培养环境中，多能细胞的特性会按顺序发生变化。之前已经描述了多能性的两个阶段，称为naive和primed。在这篇文章中，第三阶段，称为formative多能性，被提议作为naive阶段和primed阶段之间的发展连续体的一部分而存在。formative阶段被认为能够执行多能性，重塑转录、表观遗传、信号传导和代谢网络，以构成多谱系能力和对分化线索的反应。
笔记
